Efficacy of lemongrass and citronella essential oils in combination with quaternary ammonium compound against biofilm culture of Pseudomanas aeruginosa

Date

4-2014

Degree

Bachelor of Science in Biology

Major Course

Major in Microbiology

Adviser/Committee Chair

Arlene L. Llamado

Co-adviser

Fides Z. Tambalo

Abstract

The efficacy of lemongrass and citronella essential oils in combination with QUAT against biofilm formation of four Pseudomonas aeruginosa isolates was determined. The minimum inhibitory concentration (MIC) noted for both citronella and lemongrass essential oils were 1.000% while 0.030% for QUAT. The MIC values reduced the biofilm formed by P. aeruginosa B5 as observed in the crystal violet assay and confirmed by microscopy. For established biofilm, biofilm inhibitory concentration (BIC) and biofilm eradication concentration (BEC) were determined. The BIC value which inhibited the growth of established biofilm was 2.000% for both citronella and lemongrass essential oils, while 0.015% for QUAT. For complete eradication of the viable cells, the BEC values obtained were 4.000% for both citronella and lemongrass essential oils while 0.060% for QUAT. Synergistic activity was also observed against pre-formed biofilm for the combination of 0.250% citronella and lemongrass essential oil with 0.008% QUAT. For established biofilm, a combination of 1.000% essential oils and 0.030% QUAT was found to eradicate established biofilm of P. aeruginosa. Thus, lemongrass or citronella essential oils in combination with QUAT is an effective antimicrobial agent against planktonic cells, preformed biofilms and established biofilms of P. aeruginosa.

Language

English

Call Number

LG 993.5 2014 B4 /P47

Notes

status: in process

Loc: UPLB Main Library Cataloging Section

Document Type

Thesis

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