Immobilization of Lysozyme on rice hull nanosilica and preliminary investigation on its bactericidal activity against Escherichia coli

Date

4-2014

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Marivic G. Lacsamana

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

Nanosilica was extracted from rice hull by acid leaching with 1 M HCl and calcination at 650 ℃ for 6 hours. The average particle size of the rice hull silica (RHS) based on AFM analysis is 13.68 ± 4.02 nm. The surface area, pore volume, and pore diameter of the RHS based on BET results are 236.75 ± 49.45 m2/g, 0.18 ± 0.06 cm3/g and 17.02 ± 0.01 Å, respectively. Elemental analysis of the RHS using EDX showed that silicon is the major component at 98.3 ± 1.18 %. However, some of the potassium impurities remained resulting in its off-white color. Adsorption of lysozyme on RHS was found to be optimum at pH 7.5 and at an initial lysozyme concentration of 2500 ppm based on the 97.27 ± 0.13 % lysozyme adsorbed and RHS adsorptive capacity of 59.57 ± 0.4 mg/g observed at these conditions. The lysozyme immobilized on RHS at pH 7.5 did not exhibit any antibacterial activity against E. coli using the agar well diffusion assay.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2014 C42 /P47

Document Type

Thesis

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