Immobilization of Lysozyme on rice hull nanosilica and preliminary investigation on its bactericidal activity against Escherichia coli
Date
4-2014
Degree
Bachelor of Science in Chemistry
College
College of Arts and Sciences (CAS)
Adviser/Committee Chair
Marivic G. Lacsamana
Restrictions
Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.
Abstract
Nanosilica was extracted from rice hull by acid leaching with 1 M HCl and calcination at 650 ℃ for 6 hours. The average particle size of the rice hull silica (RHS) based on AFM analysis is 13.68 ± 4.02 nm. The surface area, pore volume, and pore diameter of the RHS based on BET results are 236.75 ± 49.45 m2/g, 0.18 ± 0.06 cm3/g and 17.02 ± 0.01 Å, respectively. Elemental analysis of the RHS using EDX showed that silicon is the major component at 98.3 ± 1.18 %. However, some of the potassium impurities remained resulting in its off-white color. Adsorption of lysozyme on RHS was found to be optimum at pH 7.5 and at an initial lysozyme concentration of 2500 ppm based on the 97.27 ± 0.13 % lysozyme adsorbed and RHS adsorptive capacity of 59.57 ± 0.4 mg/g observed at these conditions. The lysozyme immobilized on RHS at pH 7.5 did not exhibit any antibacterial activity against E. coli using the agar well diffusion assay.
Language
English
Location
UPLB Main Library Special Collections Section (USCS)
Call Number
LG 993.5 2014 C42 /P47
Recommended Citation
Peralta, Erle Angelo M., "Immobilization of Lysozyme on rice hull nanosilica and preliminary investigation on its bactericidal activity against Escherichia coli" (2014). Undergraduate Theses. 2035.
https://www.ukdr.uplb.edu.ph/etd-undergrad/2035
Document Type
Thesis