Isolation, purification, and characterization of a bioactive constituent of calamansi [xCitrofortunella microcarpa (Bunge) Wijnands] seeds

Date

5-2013

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Marlon N. Manalo

Restrictions

Restricted: Not available to the general public and to those bound by the confidentiality agreement. Access is available only after consultation with author/thesis adviser.

Abstract

A compound of calamansi with potential insecticidal activity was isolated by solvent extraction and column chromatography. No-choice assay of the isolate against third-instar Ostrinia furnacalis larvae showed a maximum of 10% antifeedant index at 2.5 mg per gram of insect diet. Growth inhibition index of the isolate, however, was not significantly different compared to that of the solvent and negative control. Three days after treatment, at the same concentration, the isolate exhibited corrected percent mortality three times smaller than what was observed for tebufenozide. Ehrlich's test, together with infrared (IR) and nuclear magnetic resonance (NMR) spectroscopy, suggest that the isolated compound was a limonoid.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2013 C42 /V56

Document Type

Thesis

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