Antimicrobial properties of locally isolated actinomycetes against selected bacteria and fungi

Date

2011

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ida F. Dalmacio

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Abstract

Because of continued development of bacterial resistance to antimicrobial substances and the increasing emergence of fungal infections in the Philippines, the search for novel antibiotic compounds should always be a research priority. Actinomycetes are known to naturally produce antimicrobial substances inhibitory to bacteria and fungi. To help solve the problem of bacterial and fungal infections, 55 locally isolated actinomycetes were screened for antimicrobial activity against selected test organisms, namely, Serratia marcescens, Enterobacter aerogenes, Trichophyton rubrum, Microsporum canis, and Rhizopus oryzae. A primary screening method, specifically the agar plug assay, and a secondary screening procedure, the cylinder cup assay, were conducted to select the actinomycetes that can be tested for the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) for the bacterial and fungal test organisms, respectively. Results showed that JCM 04037 and SJ21F exhibited the best inhibition among the isolates. Possibly, the extract of JCM 04037 can equal or even surpass the action of the standard streptomycin used in the study in controlling the growth of S. marcescens and E. aerogenes if further purification can be done on the crude extract. The crude extract of SJ21F had the same MFC as the cycloheximide used as standard in the assay in controlling the growth of M. canis. Bioautography experiments revealed that JCM 04037 can inhibit S. marcescens, T. rubrum, and M. canis. However, SJ21F did not show activity against the above test organisms.

Language

English

LC Subject

Actinobacteria

Location

UPLB Main Library Special Collections Section (USCS)

Document Type

Thesis

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