Parametric study on the effect of nitrogen deprivation and varying PH levels to the growth and oil production of two different strains of Chlorella vulgaris cultivated outdoors for biodiesel production

Date

2011

Degree

Bachelor of Science in Chemical Engineering

College

College of Engineering and Agro-Industrial Technology (CEAT)

Adviser/Committee Chair

Denise Ester O. Santiago

Co-adviser

Milagrosa M. Goss

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Abstract

Growth of two different strains of Chlorella vulgaris in polyethylene photobioreactors with varying Nitrogen content and pH levels was studied. It was done to determine the effect of Nitrogen deprivation in the growth and oil production of the two C. Vulgaris strains. Effect of pH level was studied to determine the range of pH the strains can be cultivated. Air was supplied by the compressor whereas sunlight was used as source of light for photosynthesis. CV3 had higher growth rate than CV1 but the latter produced higher oil yield in all cultivation conditions. CV3 had more favourable growth in the media with full nitrogen than with the low nitrogen content. This is based on the first and second specific growth rates and doubling time, which are 1.9998/day, 1.839/day and 0.447 days respectively. CV1 also had faster growth in the media with full nitrogen than with the nitrogen deprived media. This is based on the first and second specific growth rates and doubling time, which are 1.7726/day, 0.8613/day and 2.2 days respectively. In both strains, the cultures at pH 10 had faster growth rate than the cultures at pH 7 and no significant increase in biomass in cultures at pH 4. Oil yield in the n-deprived culture were 13.06% for CV1 and 7.77% for CV3 which were proven to be significantly higher than the oil yield in the n-sufficient culture with only 5.46% and 3.72% for CV1 and CV3, respectively. On the other hand no significant growth rate decrease with decrease in nitrogen content.

Language

English

LC Subject

Biodiesel fuels, Nitrogen, Chlorella vulgaris

Location

UPLB College of Engineering and Agro-Industrial Technology (CEAT)

Call Number

LG 993.5 2011 E62 B33

Document Type

Thesis

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