Genetic relationship of commercial varieties of tomato (Solanum lycopersicum L.) in the Philippines based on mapped molecular markers and disease response against tomato leaf curl Philippine virus

Date

4-2014

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Haydee F. Galvez

Abstract

Abstract: A total of 30 tomato accessions mainly commercial varieties, breeding lines and local varieties were used in this study. Major Ty-genes (Ty-1, Ty-2 and Ty-3), which confer resistance to Tomato yellow leaf curl virus (TYLCV), were screened and correlated to disease response of the test plants to Tomato leaf curl Philippine virus (ToLCPV). A breeding line, harboring Ty-1, Ty-2 and Ty-3, was the only resistant tomato; two tomato varieties were susceptible and the rest were all highly susceptible. In the genetic diversity analysis, 12 SSR markers mapped from each tomato chromosome were analyzed. Based on Simple Matching (SM) similarity coefficient and UPGMA clustering, the genetic similarity among the tomato test varieties ranged from 0.60 to 0.95. At 0.75 similarity coefficient, five cluster groupings could be classified that clearly separated Solanum pimpinellifolium from S. lycopersicum tomato. Furthermore, the highest genetic similarity (0.95 SM) was observed between two varieties developed by the same local breeding institution. This implies share of highly similar genetic materials in the development of these two tomato varieties.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2014 A127 /D36

Document Type

Thesis

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