Comparative analysis of the tensile strength and bunchy top virus resistance of parentals and backcrosses of abaca (Musa textilis Nee) hybrids and development of DNA markers tagging virus-host factors in abaca

Date

4-2014

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Antonio G. Lalusin

Abstract

Twenty-five plant samples from three generations of backcrossses of abaca hybrids and their parents were evaluated for their tensile strength, agronomic traits, and bunchy top virus resistance as a research internship to be familiarized with techniques in modern biotechnology. Using molecular marker analysis, all abaca hybrids were found to contain the gene sequences for resistance from the donor parent, Pacol. Recurrent parent genome was also recovered in some of the abaca hybrids due to similarities in banding patterns produced by the primers used. Most agronomic traits (except for leafsheath number and girth measured at the top) and tensile strength among the abaca hybrids and parents were shown to have significant differences. With respect to both parents, abaca hybrids are found to be significantly different. Three host factor genes, PCNA, Retinoblastoma, and RAD54 were selected for DNA marker development using bioinformatics tools. One set of PCR primer was designed for each gene.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993 2014 A127 M45

Document Type

Thesis

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