n-vitro assay of cytokinin-containing malunggay (Moringa oleifera Lam.) leaf extract using carrot (Daucus carota L.) root discs

Date

4-2014

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Evalour T. Aspuria

Abstract

Abstract: In-vitro effects of cytokinin-containing malunggay (Moringa oleifera Lam.) leaf extract (MLE) added in culture medium inoculated with carrot discs were quantitatively analysed and evaluated. Ten grams of young and mature malunggay leaves were extracted using 40 ml 80% ethanol. It was quantified via vis-spectrophometry and was applied in carrot disc cultures inoculated onto MS medium supplemented singly or in combination with 2,4-D. Results showed that mature malunggay leaf extract (MMLE) obtained 0.563 μgml-1 of cytokinin which is higher than 0. 030 μgml-1 of young malunggay leaf extract (YMLE). MLE also showed high antibacterial properties than its antifungal properties. Induction and growth of callus in response to media formulations were measured. Highest percentage of callus formation was observed in cultures inoculated onto MS + MMLE while lower percentage of callus formed was observed in cultures supplemented with 2,4-D in combination with MLE (young or mature). Addition of MLE alone induced cell division and formed green compact callus while addition of 2,4-D induced clear to yellow friable callus. However, few to moderate callus was obtained. Also, culture medium supplemented with MLE significantly increased the weight and size of callus. MLE (young or mature) supplemented alone promotes growth than when combined with 2,4-D.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2014 A127 S55

Document Type

Thesis

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