Histolocalization of alkaloids in cotyledon- and hypocotyl-derived calli of bittergourd (Momordica charantia L. cv. 'Makiling')

Date

4-2014

Degree

Bachelor of Science in Agriculture

Major Course

Major in Horticulture

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Evalour T. Aspuria

Abstract

Cotyledon explants of bittergourd (Momordica charantia L. cv.'Makiling') were sterilized using different sterilization procedures. It was observed that using 5% Sodium Hypochlorite (NaOCl) following the double sterilization procedure (7:7minutes) had the lowest contamination rate (18%) two weeks after inoculation. Cotyledon and hypocotyl was obtained from pre-germinated seeds of bittergourd inoculated onto MS basal medium supplemented with 1 mg/l benzylaminopurine (BAP) in combination with 0.5 mg/l indole acetic acid (IAA). Calli were produced after 7 days of incubation at the proximal end of the cotyledon and hypocotyl indicating the presence of actively dividing cells. Histological study of callus cultures from different shoot induction media formulations was conducted. Differentiating buds and bud initials were located on the surface region of the callus sections. Histolocalization of alkaloids in cotyledon and hypocotyl calli was detected using Bouchardat?s reagent. Brown precipitates were observed indicating the presence of alkaloids. Alkaloids were found to accumulate around the newly organizing tissues (buds) and distributed along the different regions of the callus. Incidentally, shoots formed in some cotyledon callus cultures inoculated onto callus induction medium. These shoots were also used for the histolocalization of alkaloids in organized tissues. Alkaloids were localized in the vascular bundle, xylem cells in particular, and in the palisade and spongy parenchymatous cells of the leaf. In this study, alkaloids can be found in different regions of cotyledon and hypocotyl callus, and stem and leaf derived from the shoots that formed from cotyledon callus inoculated onto callus induction medium.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2014 A3 P75

Document Type

Thesis

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