Cell growth stoichiometry of Pediococcus acidilactici 3G8 using distillery slops and coconut paring meal extract as substrates.

Date

2013

Degree

Bachelor of Science in Chemical Engineering

Major Course

Major in Sugar Technology

College

College of Engineering and Agro-Industrial Technology (CEAT)

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

Distillery slops and coconut paring meal are abundant by products with minimum utilization. The growth of Pediococcus acidilactici 3G8 in media with varying proportions (%v/v) of these byproducts as alternative substrates was tested in flask fermentation. The responses were examined for cell growth, substrate depletion and pH change. The first level consisted of varying proportions of distillery slops with 30% (v/v) coconut paring meal extract. The highest cell count produced was 9.14±0.10 from the 1.2% (w/v) distillery slops. The second level consisted of varying proportions of coconut paring meal extract with 1.2%(w/v) distillery slop. The highest cell count produced was 9.43±0.09 from the 2.625%(w/v) coconut paring meal. A pH decrease and no trend for substrate depletion were observed. From the flask fermentation runs, acceptable proportions of the substrates were combined in a bioreactor. Sampling was done every 2 hours for 24 hours where biomass production and substrate consumption were examined. Exponential phase was observed at 4ᵗʰ hour to 10ᵗʰ hour fermentation with a decrease in total sugar concentration. The maximum specific growth rate and doubling time of P.acidilactici 3G8 were 0.172±0.06 h⁻¹ and 4.32±1.56h, respectively.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2013 E62 P36

Document Type

Thesis

This document is currently not available here.

Share

COinS