Effect of low levels of lindane in-ovo on the embryonic growth and development of mallard ducks (Anas platyrhynchos domesticus Linn).

Date

4-2011

Degree

Bachelor of Science in Agriculture

Major Course

Major in Animal Science

College

College of Veterinary Medicine (CVM)

Adviser/Committee Chair

Vega, Renato SA

Abstract

The study was primarily designed to test in-ovo procedure for assessing the critical levels of specific organochlorines that affects the embryonic growth and development of Philippine mallard ducks. Fertile eggs at day 0 (n=200) were injected with varying concentrations of β-Benzehexachlorocyclohexane (β-BHC), diluted in a sterilized olive oil. The experiment was divided into five treatments, the negative control (T1), sham-injected (T2), 150 ppb (T3), 300 ppb (T4) and 600 ppb (T5) of β-BHC, administered in 50 µl solution. The embryonic weight, body length and head diameter were measured on day 7, 14, 21 and 28, respectively. At day 28 hatchability, movement and feeding behaviors were documented using EOS 400D® DSLR camera (Japan).) The embryo manifested a significant decrease in head diameter, body weight and body length at 600 ppb concentration. Both T4 and T5 hatched ducklings showed splay legs abnormality. Ducklings exposed to 600 ppb β-BHC were two days delayed in hatching, because of splay leg abnormality. The SDS-PAGE of plasma revealed that the 220 kDa putative vitellogenin was present in all of the treatments including control treatments indicating exposure of all layers to EDC’s.

Language

English

Location

UPLB College of Veterinary Medicine

Call Number

Thesis

Document Type

Thesis

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