Parametric study on the enzymatic hydrolysis of cellulose and hemicellulose from Sargassum sp. for bioethanol production.
Date
2012
Degree
Bachelor of Science in Chemical Engineering
College
College of Engineering and Agro-Industrial Technology (CEAT)
Restrictions
Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.
Abstract
The enzymatic hydrolysis of cellulose and hemicelluloses from Sargassum species was studied considering the enzyme loading, reaction temperature, and reaction time. It was determined that the most favorable condition is the hydrolysis at 0.5mI enzyme/g cellulose, 50ºC, and 72 h. The effect of a pretreatment process, particularly, the dilute acid hydrolysis, on the reducing sugar yield after the enzymatic hydrolysis was also determined. At the best condition,four grams of treated substrate yielded 2.474mg/mL reducing sugar, whereas, four grams of the raw substrate yielded 3.625mg/mI reducing sugar. Since the yield was higher in the untreated substrate, it was deduced that the dilute acid hydrolysis as a pretreatment for the saccharification of macroalgae can be eliminated. The theoretical ethanol yield at 90% conversion of the reducing sugar from the treated and untreated substrates was 1.4601%v/v and 2.1394%v/v, respectively.
Language
English
Location
UPLB College of Engineering and Agro-Industrial Technology (CEAT)
Call Number
Thesis
Recommended Citation
Uy, Rachelle P., "Parametric study on the enzymatic hydrolysis of cellulose and hemicellulose from Sargassum sp. for bioethanol production." (2012). Undergraduate Theses. 3231.
https://www.ukdr.uplb.edu.ph/etd-undergrad/3231
Document Type
Thesis
