Parametric study on the pretreatment of banana peelings (Musa sapientum) using dilute sulfuric acid prior to ethanol production by Saccharomyces cerevisiae

Date

2010

Degree

Bachelor of Science in Chemical Engineering

College

College of Engineering and Agro-Industrial Technology (CEAT)

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Abstract

A parametric study on the batch pretreatment of banana peelings, Musa sapientum, using dilute sulfuric acid, prior to ethanol production by Saccharomyces cerevisiae, was carried out on a laboratory scale. Samples were collected dried, milled and screened before characterization. Banana peelings were found to have the following composition: 25.1883% alpha cellulose and 15.7568% hemicellulose. Chemical pretreatment was done using 1%, 3%, and 5% v/v sulfuric acid at 50, 75, and 100ºC for 15, 30 and 45 minutes. The filtrate was analyzed for total sugar content before discarding and the pretreated biomass was used in the simultaneous saccharification and fermentation process at its best conditions. possible sugar loss on the filtrate was determined using phenol-sulfuric method. Severity of the pretreatment condition was directly related to sugar loss. The highest sugar loss was obtained at 5% acid concentration 100ºC for30 minutes which was 26.533mg/mL.The best pretreatment conditions for the banana peelings were obtained using Response Surface Methodology. Quadratic model was chosen in order to predict the effects of the three parameters on the response. Using numerical approach, the desirable pretreatment combination that produced the maximum total sugar was:1.96% sulfuric acid concentration, 69.35ºC reaction temperature for 22.99 minutes reaction time. Using the best pretreatment conditions for banana peelings ethanol yield after six days of fermentation at room temperature was 3.2g/L solution or 0.40% v/v with 60.6% cellulose conversion. Banana peelings as feedstock were capable of producing ethanol using SSF.

Language

English

LC Subject

Ethanol, Saccharomyces cerevisiae, Fermentation, Cellulose, Banana

Location

UPLB College of Engineering and Agro-Industrial Technology (CEAT)

Call Number

LG 993.5 2010 E62 O23

Document Type

Thesis

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