Optimization of enzymatic extraction of pectin from waste cacao pod husk
Date
6-2015
Degree
Bachelor of Science in Chemical Engineering
College
College of Engineering and Agro-Industrial Technology (CEAT)
Adviser/Committee Chair
Catalino G. Alfafara
Restrictions
Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.
Abstract
Enzymatic extraction of pectin from cacao pod husk was conducted in an attempt to produce useful product from this waste. Cellulase (Sigma C-9748) enzyme was used to speed up the breaking of cellulose for easier extraction of pectin. Factorial experiments showed that three factors (cellulase loading, solids loading and reaction time) were significant for the responses (pectin yield and galacturonic acid level). Box-Benhken Design results gave statistically significant quadratic models for all the responses. The optimum conditions were: 2.003 % (w/v) cellulase loading, 3.065% (w/w) cellulase loading, and 17.998 hours reaction time. The predicted pectin yield is 20.997% (w/w) and the galacturonic acid level is 207.542 mg/g. The optimum conditions were experimentally verified giving pectin yield of 20.48% w/w and galacturonic acid level of 206.72 mg/g. The experimental and numerical results were compared and the percent difference is 2.46% and 0.39% for pectin yield and galacturonic acid level respectively.
Language
English
Location
UPLB College of Engineering and Agro-Industrial Technology (CEAT)
Call Number
LG 993.5 2015 E62 /P36
Recommended Citation
Penisa, Xaviery N., "Optimization of enzymatic extraction of pectin from waste cacao pod husk" (2015). Undergraduate Theses. 3981.
https://www.ukdr.uplb.edu.ph/etd-undergrad/3981
Document Type
Thesis