Optimization of enzymatic extraction of pectin from waste cacao pod husk

Date

6-2015

Degree

Bachelor of Science in Chemical Engineering

College

College of Engineering and Agro-Industrial Technology (CEAT)

Adviser/Committee Chair

Catalino G. Alfafara

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

Enzymatic extraction of pectin from cacao pod husk was conducted in an attempt to produce useful product from this waste. Cellulase (Sigma C-9748) enzyme was used to speed up the breaking of cellulose for easier extraction of pectin. Factorial experiments showed that three factors (cellulase loading, solids loading and reaction time) were significant for the responses (pectin yield and galacturonic acid level). Box-Benhken Design results gave statistically significant quadratic models for all the responses. The optimum conditions were: 2.003 % (w/v) cellulase loading, 3.065% (w/w) cellulase loading, and 17.998 hours reaction time. The predicted pectin yield is 20.997% (w/w) and the galacturonic acid level is 207.542 mg/g. The optimum conditions were experimentally verified giving pectin yield of 20.48% w/w and galacturonic acid level of 206.72 mg/g. The experimental and numerical results were compared and the percent difference is 2.46% and 0.39% for pectin yield and galacturonic acid level respectively.

Language

English

Location

UPLB College of Engineering and Agro-Industrial Technology (CEAT)

Call Number

LG 993.5 2015 E62 /P36

Document Type

Thesis

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