Parametric study on mixotrophic cultivation of Chlorella vulgaris Beijerinck for biodiesel production

Date

7-2015

Degree

Bachelor of Science in Chemical Engineering

College

College of Engineering and Agro-Industrial Technology (CEAT)

Adviser/Committee Chair

Rex B. Demafelis

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

Chlorella vulgaris CV1 strain isolate from Laguna de Bay were investigated for biomass productivity by mixotrophic cultivation by manipulating four variables: illumination patterns (natural or artificial light cycle), carbon source (glucose or glycerol), carbon source concentration (0.5 or 10 gL^-1) and nitrogen source (urea) concentration (0.02073 or 0.04146 gL^-1) in 100-mL cultures. Using dry cell weight method, it was found out that the largest biomasss productivity was obtained from the microalgal culture treated with 10gL^-1 glycerol, 0.04146 gL^-1 urea and under controlled light. Furthermore, it was observed that there is significant difference in the biomass productivities at different carbon sources (favoring glycerol) while no difference were observed at different carbon concentrations, urea concentrations and illumination patterns. The cultivation conditions at best biomass productivities were cultivated in two 4-L cultures for biomass harvesting and oil extraction. The oil yield and lipid productivity was found to be 3.50% and 7.5042 mgL^-1day^-1. The lipid obtained was found to contain 46.19% triacylglycerol (TAG), 47.26% free-fatty acids (FFA) and 6.555% diacylglycerols (DAG). The high FFA conteny has made the Chlorella vulgaris oil unsuited for transesterification process.

Language

English

Location

UPLB College of Engineering and Agro-Industrial Technology (CEAT)

Call Number

LG 993.5 2015 E62 /O47

Document Type

Thesis

This document is currently not available here.

Share

COinS