Parametric study on mixotrophic cultivation of Chlorella vulgaris Beijerinck for biodiesel production
Date
7-2015
Degree
Bachelor of Science in Chemical Engineering
College
College of Engineering and Agro-Industrial Technology (CEAT)
Adviser/Committee Chair
Rex B. Demafelis
Restrictions
Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.
Abstract
Chlorella vulgaris CV1 strain isolate from Laguna de Bay were investigated for biomass productivity by mixotrophic cultivation by manipulating four variables: illumination patterns (natural or artificial light cycle), carbon source (glucose or glycerol), carbon source concentration (0.5 or 10 gL^-1) and nitrogen source (urea) concentration (0.02073 or 0.04146 gL^-1) in 100-mL cultures. Using dry cell weight method, it was found out that the largest biomasss productivity was obtained from the microalgal culture treated with 10gL^-1 glycerol, 0.04146 gL^-1 urea and under controlled light. Furthermore, it was observed that there is significant difference in the biomass productivities at different carbon sources (favoring glycerol) while no difference were observed at different carbon concentrations, urea concentrations and illumination patterns. The cultivation conditions at best biomass productivities were cultivated in two 4-L cultures for biomass harvesting and oil extraction. The oil yield and lipid productivity was found to be 3.50% and 7.5042 mgL^-1day^-1. The lipid obtained was found to contain 46.19% triacylglycerol (TAG), 47.26% free-fatty acids (FFA) and 6.555% diacylglycerols (DAG). The high FFA conteny has made the Chlorella vulgaris oil unsuited for transesterification process.
Language
English
Location
UPLB College of Engineering and Agro-Industrial Technology (CEAT)
Call Number
LG 993.5 2015 E62 /O47
Recommended Citation
Ofrasio, Bjorn Ivan G., "Parametric study on mixotrophic cultivation of Chlorella vulgaris Beijerinck for biodiesel production" (2015). Undergraduate Theses. 3985.
https://www.ukdr.uplb.edu.ph/etd-undergrad/3985
Document Type
Thesis