Parametric study on enzymatic pretreatment of napier grass (Pennisetum purpureum Schumach) leaves for bioethanol production

Date

6-2015

Degree

Bachelor of Science in Chemical Engineering

College

College of Engineering and Agro-Industrial Technology (CEAT)

Adviser/Committee Chair

Myra G. Borines

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

Napier grass (Pennisetum purpureum Schumach) was chosen for this study as the feedstock for bioethanol production. Enzymatic pretreatment was used because of its low energy demand and minimal waste production. A two-factorial experiment was performed to determine the effects of laccase (mL/g substrate), xylanase loading (mL/g substrate), pH, and temperature on the reducing sugar yield (mg/g) after pretreatment and enzymatic hydrolysis. Results from the pretreatment showed that xylanase loading, temperature, and laccase loading have significant effects on the reducing sugar yield. The highest yield of reducing sugar was 168.33 mg/g biomass obtained after 4 h of enzymatic pretreatment with 1.46 mL xylanase/g sample (xylanase volumetric activity of 479.3 U/mL), Ph 4, at 30ºC. The biomass after pretreatment was subjected to enzymatic hydrolysis with 0.61 mL cellulase/g sample loading (cellulase volumetric activity of 1156.5U/mL0, 2.5% (w/v) solid concentration, at 50ºC for 72 h. Results showed that xylanase loading and temperature have significant effects on the reducing sugar yield after enzymatic hydrolysis. The highest yield of reducing sugar was 173.430 mg?g biomass obtained after 72 h of enzymatic hydrolysis of enzyme- pretreated biomass with 1.46 m,L xylanase/g sample, pH 4, at 30ºC. The highest yield of total reducing sugar was 341.76 mg/g biomass obtained after enzymatic pret5oreatment and hydrolysis significantly improved the production of fermentable sugars for bioethanol production.

Language

English

Location

UPLB College of Engineering and Agro-Industrial Technology (CEAT)

Call Number

LG 993.5 2015 E62 /M31

Document Type

Thesis

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