Optimization and scale-up studies for the fermentative production of phosphate solubilizing bacteria in stirred tank bioreactors using molasses and whey as medium components

Date

6-2015

Degree

Bachelor of Science in Chemical Engineering

College

College of Engineering and Agro-Industrial Technology (CEAT)

Adviser/Committee Chair

Catalino G. Alfafara

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

The study attempted to optimize both an alternative medium (containing molasses and unsalted whey) as well as the aeration/agitation conditions (in a 5L stirred tank bioreactor) for the production of phosphate solubilizing bacteria (PSB) via Response Surface Methodology (RSM). The optimum aeration/agitation conditions of the 5-L bioreactor were then used to calculate the volumetric oxygen mass transfer coefficient (kLa), for use as a scale-up factor for scaling the process to a 30-L pilot scale reactor. Finally, the reproducibility of fermentation performance was verified in the 30-L bioreactor. The optimum alternative media conditions for maximum biomass was found to be at an initial sugar concentration of 6.29 mg/mL and a carbohydrate-to-protein ratio equal to 0.05. The experimentally verified optimum aeration/agitation conditions in the 5-L bioreactor was 233rpm agitation speed and 1.22vvm aeration rate. The corresponding kL¬a at optimum conditions was 0.00411 s-1. The reproducibility of the optimum fermentation performance in the scaled 30-L bioreactor was successfully achieved in verification experiments. The combination of statistical and biochemical engineering approaches were shown to be a useful strategy for optimization and scale-up for the production of phosphate solubilizing bacteria.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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