Antibiotic resistance patterns of non-intestinal Escherichia coli in chickens from commercial farms in CALABARZON

Date

6-2015

Degree

Doctor of Veterinary Medicine

College

College of Veterinary Medicine (CVM)

Abstract

Fifteen (15) Extra-intestinal Pathogenic E. coli (ExPEC) were isolated from layer chickens (n=72) and 20 revived ExPEC (Rama, 2014) samples from broiler chickens (n=106) were used to analyze their antimicrobial resistance patterns against common antibiotics used in commercial poultry farms in CALABARZON, Philippines. These were tested using the disk diffusion and the Minimum Inhibitory Concentration (MIC) determination. Antimicrobial genes of public health concern included Extended Spectrum Beta Lactamases (EBSL), carbapenemases and AmpC beta lactamases were detected. The E.coli isolates were from the respiratory tract (infraorbital sinus, trachea and air sac) and tissue impressions (lungs, spleen, pericardium, heart) of necropsied chickens. The highest recovery rate in layerswas in the lungs (33.33%) while in broilers, it was the spleen (23.1%). Both disk diffusion and MIC revealed that resistance was higher in broilers than in layers.E. coli from broilers had highest resistance to four antibiotics of human importance- ampicillin, chloramphenicol, TMPS, and ciprofloxacin while E. coli from layers showed highest resistance to ampicillin. All E. coli were susceptible to ceftriaxone (3rd generation antibiotic). Disk diffusion method was performed twice, using 17 antimicrobials of veterinary importance while the second only used 6 of public health interest. Differences in protocols followed and laboratory testing sites are the main reasons for variabilities. The second detection classified more resistant organisms than the first test.Significant statistical difference in the determination of resistance for ampicillin, chloramphenicol, gentamycin and TMPS was observed. No resistance genes for ESBL and carbapenemase were detected but a positive AmpC beta lactamase isolate from layer was detected.

Language

English

Location

UPLB College of Veterinary Medicine (CVM)

Call Number

LG 993.5 2015 V4 A23

Document Type

Thesis

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