Efficacy of topical Origanum vulgare L. crude leaf extract in experimentally induced skin wounds in ICR mice (Mus musculus Linn.)

Date

6-2015

Degree

Doctor of Veterinary Medicine

College

College of Veterinary Medicine (CVM)

Adviser/Committee Chair

Marco F. Reyes

Co-adviser

Rey B. Oronan

Abstract

A total of 24 ICR adult mice of both sexes, were randomly assigned into four treatment groups: NG (0.9% NaCl Solution), MG (5% Mupirocin ointment), DG (Oregano decoction) and PG (Oregano poultice) to evaluate the efficacy of Origanum vulgare L. in the healing of incisional skin wounds. Treatment was administered once daily for the duration of the study. Macroscopic characterization was done on days seven, 14, and 21. Gross inflammation was observed in all treatment groups for up to seven days. Complete wound closure was observed by day 21: with PG having mean healing time of 14 days. The microscopic characteristics were also described on day 7 and 21. Collagen formation was observed in all treatment groups. Neovascularization and fibroblasts formation were most evident in MG and DG. Furthermore, the PG exhibited the greatest degree of epithelialization with the least inflammatory cell migration. Statistical analysis at 5% degree of significance showed that the presence of gross inflammation and neovascularization are significantly different between treatment groups. The use of Oregano as an alternative treatment to skin wounds is apparently comparable with that of conventional treatment modalities.

Language

English

Location

UPLB College of Veterinary Medicine (CVM)

Call Number

LG 993.5 2015 V4 T45

Document Type

Thesis

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