Macroscopic and microscopic characteristics of experimentally induced corneal ulcer treated with autologous serum in non descript dogs (canis familiaris)

Date

6-2015

Degree

Doctor of Veterinary Medicine

College

College of Veterinary Medicine (CVM)

Adviser/Committee Chair

Marco F. Reyes

Co-adviser

Rey B. Oronan

Abstract

Corneal ulcer, common ocular problem affecting dogs is treated with a combination of ophthalmic medications. Thisstudy was conducted to determine the effects of topicalautologous serum in the treatment of experimentally induced corneal ulcers. Corneal ulcer was induced in ten apparently healthy, adult female, non-descript dogs (Canisfamiliaris). The animals were randomly assigned into three treatment groups: A(NSS), B (antibiotics) and C (autologous serum), and received 0.5 mLtopical ophthalmic treatmenttwice daily for the duration of the study. The clinical signs of the corneal ulcer were observed and photo-documented at seven, 14, and 21 days post-operative and the animals were sacrificed on the samedaysthe corneas were processed for histopathology. Grossly, corneal edema was observed in all groups at day 7, which gradually diminisheduntil day 21.Varying degrees of conjunctival hyperemia and vascularization were most evident in A and B. Histopathological examination in all groups revealedthat corneal epithelial thickening, invagination and fibroblastic proliferation were consistent in all groups. The presence ofpolymorphonuclear cells in the epithelium was not observed in the autologous group. The use of autologous serum was also found to be more economical compared to the use of ophthalmic antibiotics.

Language

English

Location

UPLB College of Veterinary Medicine (CVM)

Call Number

LG 993.5 2015 V4 P73

Document Type

Thesis

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