Conventional polymerase chain reaction -based detection of pathogenic leptospires in dogs presented at the University of the Philippines Veterinary Teaching Hospital Diliman Station, Quezon City
Date
6-2015
Degree
Doctor of Veterinary Medicine
College
College of Veterinary Medicine (CVM)
Adviser/Committee Chair
Therese Marie A Collantes
Co-adviser
Marianne Leila S. Flores
Abstract
Diagnosis of canine leptospirosis is important at the early stage of infection when leptospires can be detected in the blood, however, infected dogs often present non-specific signs and hematological values which make it difficult to diagnose. In this study, 60 blood samples from canine patients at the University of the Philippines Veterinary Teaching Hospital (UP VTH) in Diliman, Quezon City were tested for leptospirosis using polymerase chain reaction assay. Of the 60 samples, 11 samples (18%) tested positive for pathogenic leptospires which is statistically significant based on Z ? test (α=0.05). In contrast, statistical analysis revealed no significant correlation between positive result upon PCR and the variables (age, sex, breed, type of housing and vaccination history). The top presenting signs of dogs that were positive for pathogenic leptospires were vomiting and diarrhea (7/11) while leukocytopenia (5/11) was the predominant hematologic finding. This study was able to detect leptospiral DNA in the blood of dogs and provided a clinical picture of the 11 dogs infected with pathogenic leptospires.
Language
English
Location
UPLB College of Veterinary Medicine (CVM)
Call Number
LG 993.5 2015 V4 D38
Recommended Citation
David, Joanna Marie F., "Conventional polymerase chain reaction -based detection of pathogenic leptospires in dogs presented at the University of the Philippines Veterinary Teaching Hospital Diliman Station, Quezon City" (2015). Undergraduate Theses. 4179.
https://www.ukdr.uplb.edu.ph/etd-undergrad/4179
Document Type
Thesis