Conventional polymerase chain reaction -based detection of pathogenic leptospires in dogs presented at the University of the Philippines Veterinary Teaching Hospital Diliman Station, Quezon City

Date

6-2015

Degree

Doctor of Veterinary Medicine

College

College of Veterinary Medicine (CVM)

Adviser/Committee Chair

Therese Marie A Collantes

Co-adviser

Marianne Leila S. Flores

Abstract

Diagnosis of canine leptospirosis is important at the early stage of infection when leptospires can be detected in the blood, however, infected dogs often present non-specific signs and hematological values which make it difficult to diagnose. In this study, 60 blood samples from canine patients at the University of the Philippines Veterinary Teaching Hospital (UP VTH) in Diliman, Quezon City were tested for leptospirosis using polymerase chain reaction assay. Of the 60 samples, 11 samples (18%) tested positive for pathogenic leptospires which is statistically significant based on Z ? test (α=0.05). In contrast, statistical analysis revealed no significant correlation between positive result upon PCR and the variables (age, sex, breed, type of housing and vaccination history). The top presenting signs of dogs that were positive for pathogenic leptospires were vomiting and diarrhea (7/11) while leukocytopenia (5/11) was the predominant hematologic finding. This study was able to detect leptospiral DNA in the blood of dogs and provided a clinical picture of the 11 dogs infected with pathogenic leptospires.

Language

English

Location

UPLB College of Veterinary Medicine (CVM)

Call Number

LG 993.5 2015 V4 D38

Document Type

Thesis

This document is currently not available here.

Share

COinS