Sex determination of domestic pigeons (Columba livia) based on chromo helicase DNA-binding (CHD) gene isolated from blood and feathers using polymerase chain reaction

Date

6-2015

Degree

Doctor of Veterinary Medicine

College

College of Veterinary Medicine (CVM)

Adviser/Committee Chair

Therese Marie A Collantes

Abstract

Due to the absence of sexual dimorphism among pigeons, sex determination is proven to be difficult. This study was conducted to determine the sexes of 15 mature and 4 juvenile domestic pigeons using phenotypic and genotypic methods. Phenotypic observation was performed using 15 parameters while genotypic method involved PCR assay of CHD gene isolated from blood and feathers using P2/P8 primers. Gonadal examination via necropsy was done to confirm the actual sexes of the juvenile pigeons. Phenotypic observation has 25% accuracy compared to genotypic method using blood and feathers with 100% and 50% accuracy, respectively, as compared with gonadal examination. Mature domestic pigeons were also sexed through genotypic method using blood and feathers and resulted to a 53.33% match with each other. Since sex determination using blood was proven 100% accurate in 4 birds tested, the actual sexes of the mature pigeons were based on the blood results (66.67% male and 33.33% female). Finally, it was statistically proven that sex determination based on CHD gene isolated from blood has 100% sensitivity and 100% specificity.

Language

English

Location

UPLB College of Veterinary Medicine (CVM)

Call Number

LG 993.5 2015 V4 C38

Document Type

Thesis

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