Molecular characterization of Ethidium Bromide (EtBr)-induced mutant abaca (Musa textilis Nee) for abaca bunchy top virus resistance using simple sequence repeats and gene specific markers

Date

7-2015

Degree

Bachelor of Science in Agriculture

Major Course

Major in Agronomy

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Antonio G. Lalusin

Abstract

Ninety two ethidium bromide (EtBr)-induced mutant abaca accessions putatively resistant to abaca bunchy top virus (AbBTV) were screened using eight SSR markers, three resistance gene analogs (RGA), and two gene specific markers. Band amplifications were observed only on six SSR markers and three RGAs. The high polymorphism information content (PIC) value is suggestive of the usefulness of the markers for future breeding studies of mutant abaca. Different number of bands and banding patterns were generated for each marker. Based on these observations, the number of mutant accessions was determined. The markers identified different number of mutant accessions, but only 15 accessions were found to be mutants in all the markers. The accessions were assessed for their resistance to AbBTV depending on the presence of three RGAs. All the accessions were identified as resistant due to the presence of RGA. The identified mutant accessions can be used as stocks for breeding abaca plants resistant to AbBTV.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2015 A3 /C37

Document Type

Thesis

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