Propagation of an infectious brochitis virus using a local isolate and a vaccine strain for the hemagglutination inhibition test and the characterization of specific immune sera produced in rabbits

Date

6-2015

Degree

Doctor of Veterinary Medicine

College

College of Veterinary Medicine (CVM)

Adviser/Committee Chair

Hope G Rovira

Abstract

Infectious bronchitis virus (IBV) is a common viral disease of chickens causing respiratory signs, nephritis, poor egg production and quality. In this study, an attempt was made to propagate virus and raise specific immune sera in rabbits against a local Infectious bronchitis (IB) virus isolate and Mass H120, a commercial vaccine strain. The viruses were prepared by propagation in embryonated chicken eggs where the Embryo Infective Dose 50 (EID50) of the IB field strain was 105.3 EID50/ml. Rabbits were divided into three groups A (immunized with IB vaccine strain antigen), group B (immunized with IB field strain antigen) and Group C uninoculated controls. The hemagglutination (HA) titer of the field strain antigen (homologous) was 1:2056. The antibody titer of 1:256 in group B against field strain antigen was the highest titer determined at 54 days of inoculation. While the final titer of group A (heterologous M-H120) was 1:64. The uninoculated control and pre-immune sera had a titer (highest titer of 1:128) indicating contamination of controls thus fetal bovine serum tested five times at 1:2 was used as negative control to assert baseline. Low hemagglutination inhibition titer of heterologous strains indicate low inhibiting antibodies.

Language

English

Location

UPLB College of Veterinary Medicine (CVM)

Call Number

LG 993.5 2015 V4 B87

Document Type

Thesis

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