Effects of nematophagous fungi Duddingtonia flagrans as biological control against common strongyle roundworms of buffaloes (Bubalus bubalis)

Date

6-2015

Degree

Doctor of Veterinary Medicine

College

College of Veterinary Medicine (CVM)

Adviser/Committee Chair

Therese Marie A Collantes

Co-adviser

Claro N Mingala

Abstract

Gastrointestinal nematodes are a major problem in grazing animals. Control is achieved through administration of anthelmintics, however, because of indiscriminate use there have been increased reports of its resistance. This study determined the efficacy of Duddingtonia flagrans as biological control against common strongyle roundworms of buffaloes. Using corn meal agar (CMA) assay, strongyle infective larvae were treated with and without D. flagrans. Results showed that D. flagrans group had significantly lower larvae counts than non-treated groups. The chlamydospore/gram (CG) assay tested a dose-dependent concentration wherein feces with 2,100 eggs/gram (EPG) strongyles were treated with D. flagrans increasing doses of chlamydospores/g feces (100,000, 250,000 and 500,000). Results showed an 84.39% larval reduction after treatment with 500, 000 chlamydospores/g. The chlamydspore/egg assay (CE) evaluated increasing ratios of egg to chlamydospore dose (1:0, 1:100, 1:500, 1:1000) using the 2,100 EPG feces. The ratio 1:500received the highest percent larval reduction (78.88%). D. flagrans was directly fed to buffaloes at varying concentrations (50,000, 150,000, 250,000 chlamydospores/kg BW). A 78.77% larval reduction was observed at 50,000 chlamydospore/kg BW oral administration for 5 days. This study showed the efficacy of D. flagrans as a potential alternative for anthelmintics in buffaloes in the Philippines.

Language

English

Location

UPLB College of Veterinary Medicine (CVM)

Call Number

LG 993.5 2015 V4 B37

Document Type

Thesis

This document is currently not available here.

Share

COinS