Polymorphisms in estrogen receptor gene (ESR) and their association with some sow productivity traits of large white sows (Sus scrofa L.)

Date

12-2015

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Renato SA. Vega

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

The number of weaned per sow per year is affected by litter size born, litter size at weaning (LSW) and weaning to estrus interval (WEI). Hence association of these traits to the candidate gene estrogen receptor gene (ESR) will help in selection for improved sow productivity. The study used 30 Large White sows from a breeder farm. The target ESR gene fragment was amplified using DNA from hair follicle. Genotyping was performed using PCR-RFLP. Digestion was carried out using PvuII restriction enzyme that cuts in the presence of B allele. The results showed that the A allele frequency (0.68) was higher than the B allele (0.32). Fifteen AA genotype sows (0.5), 11 AB sows (0.37), and 4 BB (0.13) sows were identified. The summary statistics showed a low value of gene diversity (0.44), heterozygosity (0.37) and Polymorphism Information Content (PIC) (0.34). Heterozygosity and gene diversity had a difference of 0.07 that implies that the gene in the population is not in Hardy-Weinberg Equilibrium (HWE). It was found out that the ESR genotypes had no significant association to LSW and WEI.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2015 A127 /B37

Notes

Major in Animal Biotechnology

Document Type

Thesis

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