Growth and phycocyanin pigment responses of Sprinula platensis Gomont BIOTECH 4012 cultivated in polyethylene photobioreactors

Date

6-2016

Degree

Bachelor of Science in Chemical Engineering

College

College of Engineering and Agro-Industrial Technology (CEAT)

Adviser/Committee Chair

Marilyn C. Del Barrio

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

Spirulina platensis, a cyanobacterium known for its nutritional value, was cultivated in improvised bubble-type polyethylene photobioreactors. The effect of varying growth parameters namely light quality, salinity, and alkalinity on the growth-related parameters (doubling time and microalgal productivity) and phycocyanin (blue pigment) yield were determined through a factorial design experiment. The experiments were performed at room temperature and aeration rate of 3.74 vvm. Analysis of Variance productivity and phycocyanin extraction yield were all statistically signifiant at p ≤ 0.05. Only the interaction between salinity and light quality has no significant effect on the doubling time. Post hoc Fisher's Least Significant Difference (LSD) and analysis of the interaction and 3D surface plots show that the most likely best condition for both growth-related parameters are green light at 1 g/L salinity and pH 13. However, high phycocyanin yield are flavored at a higher salinity (3 g/L) and lower pH (pH 9), at the different light quality of red. The results may be useful information for RSM-designed experiments to obtain the optimum conditions.

Language

English

Location

UPLB College of Engineering and Agro-Industrial Technology (CEAT)

Call Number

LG 993.5 2016 E62 /P36

Document Type

Thesis

This document is currently not available here.

Share

COinS