Effect of different equilibration time and concentration of ethylene glycol on the post-thaw morphology of frozen buffalo spermatozoa

Date

6-2016

Degree

Doctor of Veterinary Medicine

College

College of Veterinary Medicine (CVM)

Adviser/Committee Chair

Conrado A. Valdez

Co-adviser

Eduardo B. Torres

Abstract

Determination of the effect of different equilibration time and concentrations of ethylene glycol on the post-thaw morphology of frozen buffalo spermatozoa was done in this study. Pooled semen samples of four Murrah Buffaloes were diluted in Tris-yolk extenders with 7%, 8%, and 9% concentrations of ethylene glycol subjected to 20, 30, 40, and 50 minutes equilibration time. The samples were suspended in liquid nitrogen for seven minutes and then frozen and stored in a liquid nitrogen tank. After one week of storage, they were thawed in a water bath at 41℃ for 17 seconds. Samples were then stained and evaluated, counting one hundred spermatozoa and classifying under normal, or with primary, secondary, and tertiary abnormalities. Analysis showed that there was no significant interaction between the concentration of ethylene glycol and equilibration time. The results also showed that the least number of morphological abnormalities were observed in 7% concentration under 20 minutes equilibration and primary abnormalities were observed the highest. Results did not meet the limit for artificial insemination however, effectivity of freezing can only be assessed through embryo development.

Language

English

Location

UPLB College of Veterinary Medicine (CVM)

Call Number

LG 993.5 2016 V4 P36

Document Type

Thesis

This document is currently not available here.

Share

COinS