Date

2021

Degree

Doctor of Veterinary Medicine

College

College of Veterinary Medicine (CVM)

Adviser/Committee Chair

Michelle Grace V. Paraso, Ma. Suzanneth Epifania G. Lola

Abstract

This study was conducted to determine the potential effects of EDCs on juvenile male Nile tilapia. Measurement of E2 and BPA in water samples were conducted using enzyme linked immunosorbent assay (ELISA). Testis, liver, and kidneys were excised, and the gonadosomatic index (GSI), hepatosomatic index (HSI), and presence of endocrine- related histopathological lesions were determined. Results revealed highly

detectable levels of E2 and BPA in all samples, including water from the deep well supply of the UPLB Limnological Research Station. GSI was lower in fish from Calibato and Yambo lakes in comparison to Sampaloc Lake. HSI was comparatively higher in fish from Sampaloc Lake than in Yambo and Palakpakin fish. Testicular degeneration and Leydig cell hyperplasia/hypertrophy were observed in the testis. Liver samples manifested increased hepatocyte basophilia, loss of cytoplasmic vacuolization, hepatic blood vessels containing proteinaceous fluid, localized vacuolar degeneration, and perivasculitis. Kidney samples showed glomerular epithelial cell hypertrophy, vacuolar swelling, tubular epithelium necrosis, hyaline droplets within glomerular and tubular epithelium, interstitial nephritis, and possible tumors. These observations may have been caused by fish exposure to EDCs including E2 and BPA. The identification of sources of EDCs and important fish species affected by these compounds in the lakes is recommended.

Language

English

Location

UPLB College of Veterinary Medicine (CVM)

Notes

Best Thesis in Veterinary Public Health, 2021

Document Type

Thesis

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