Susceptibility of clinical Candida isolates to garlic extracts and determination of the minimum inhibitory and fungicidal concentrations.

Date

4-2013

Degree

Bachelor of Science in Biology

Major Course

Major in Microbiology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Cruz, Wilma T

Co-adviser

Flores, Leah B

Abstract

The susceptibility of seven putative Candida isolates recovered from the vaginal swab specimens of women patients of Laguna Provincial Hospital to different garlic extracts was studied. Through observation of cultural characteristics of CHROMAgar Candida and morphology on Cornmeal Agar with 2% Dextrose, four isolates were presumptively identified as C. albicans while three were classified as C. glabrata. The anticandidal susceptibility to aqueous (AGE), heated (HGE), ethanolic (EGE), ethanolic (EGE), and methanolic (MGE) garlic extracts were was assed using the Kirby Bauer method disk-diffusion assay. Aqueous garlic extract had the highest mean zones of inhibition (ZOI) against all seven clinical isolates and control organism C. krusei, with a range of 29 mm to 35 mm. The mean ZOI values of HGE (range = 23-31 mm), EGE (range = 25-31 mm), and MGE (range = 6-17 mm) were significantly lower compared to the ZOI values of AGE. Variation in susceptibility to the extracts between species was noted. Using the microboth dilution method and subsequent planting, the minimum inhibitory (MIC) and fungicidal (MFC) concentrations of the extract that exhibited the highest inhibition zone diameters against each of the isolates (AGE) were determined. Aqueous garlic extract was both inhibitory and fungicidal between 16 and 32 ug/mL. Significant variations in the MIC and MFC values between species were not observed.

Language

English

Location

UPLB Main Library Special Collections Section

Call Number

Thesis

Document Type

Thesis

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