Prokaryotic community structure of deep litter bed as revealed by PCR-DGGE analysis and isolation of an antibiotics-producing actinomycetes

Date

4-2013

Degree

Bachelor of Science in Biology

Major Course

Major in Microbiology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Opulencia, Rina B.

Abstract

The study was conducted to determine the diversity of prokaryotes in deep litter bed samples obtained from pig and chicken confinement facilities. The total genomic DNA was extracted from each sample, which were used to amplify the 16S rDNA of the prokaryotic species followed by amplification of the hyper variable region. The amplicons were subjected to DGGE to determine the diversity of Bacteria and Archaea in each sample. There is no significant difference in prokaryotic diversity as measured by Simpson's index of diversity and Shannon-Weiner index between the two samples, indicating that source of litter, from pig or from chicken, does not greatly affect in diversity of the prokaryotes. However, Bacteria were found to have higher Simpson's index of diversity and Shannon-Weiner index values in the two samples than Archaea. This would mean that, based on the methods used, the environment favors the growth of Bacteria than Archaea and they were the major participant in the processes occurring in the environment, particularly in decomposition. For the isolation of antibiotics-producing actinomycetes, the isolates were subjected to primary and secondary screening for antibiotics production, tested against gram-positive bacteria, Bacillus subtilis and Staphylococcus aureus, and gram negative bacteria, Escheria coli and Proteus vulgaris. Seven out of eleven isolates were positive for primary screening and two were positive in secondary screening.

Language

English

Location

UPLB Main Library Special Collections Section

Call Number

Thesis

Document Type

Thesis

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