Safety evaluation of nanomaterial, polymer and diluent intended for nanocomposite coatings of fruits and vegetables using female ICR mice : physiology, organopathy and histopathology

Date

6-2016

Degree

Doctor of Veterinary Medicine

College

College of Veterinary Medicine (CVM)

Adviser/Committee Chair

Maria Amelita C. Estacio

Co-adviser

Therese Marie A. Collantes

Abstract

An acute oral toxicity test of polymer, nanomaterials and diluent was done to evaluate their safety as nanocomposite coatings to fruits and vegetables. Body weight, feed and water intake were not negatively affected of the treatments whereas docile behavior, weakness, hypothermia, dyspnea were apparent in pectin, 1% acetic acid, chitosan tripolyphosphate and cellulose nanowhiskers group. Treatment related mortality was few. Grossly, splenomegaly with or without necrotic foci in all groups except distilled water, focal intestinal hemorrhage in pectin and distilled water and dark discoloration in the liver in chitosan TPP, were noted. Histopathologically, basophilic aggregates from esophagus to stomach in all groups, intestinal degeneration and lymphatic nodule micronecrosis in distilled water, 1% acetic acid and pectin, enlarged lymphatic nodule in pectin, increased mucin production in enlarged goblet cells in hemicellulose, pectin and chitosan TPP, renal and hepatic necrosis and degeneration in all groups, brain lesions in chitosan TPP, cellulose nanowhiskers and cellulose nanorods, splenic lesions in hemicellulose, pectin and chitosan TPP, and renal dark substances in pectin, chitosan TPP, cellulose nanowhiskers and cellulose nanorods were observed. Findings suggest that ingestion of nanomaterial, polymer and diluent cause abnormalities in behavior, gross and histopathological features of selected organs in female ICR mice.

Language

English

Location

UPLB College of Veterinary Medicine (CVM)

Call Number

LG 993.5 2016 V4 N53

Document Type

Thesis

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