Phylogenetic analysis of the hypervariable region of the fusion (F) gene of Newcastle disease virus from commercial layer farms in several provinces in the Philippines

Date

6-2016

Degree

Doctor of Veterinary Medicine

College

College of Veterinary Medicine (CVM)

Adviser/Committee Chair

Dennis V. Umali

Abstract

Newcastle disease (ND) is a fatal disease affecting poultry flocks all around the world that continues to cause high mortality rates and severe economic losses in the poultry industry. Samples from five field cases of ND from commercial layer flocks located in different provinces in the Philippines, specifically in Pampanga, Bulacan, Zamboanga del Sur and Davao, were processed to confirm infection of ND. Newcastle disease virus (NDV) isolates were amplified through RT-PCR and studied through nucleotide sequencing and phylogenetic analysis. The genetic characteristics of all five Philippine NDV isolates confirmed that they were virulent with the cleavage site motif of 112RRRKR*F117 and 112RRQKR*F117. Phylogenetic analysis illustrated that the NDV strains belonged to class II genotype VII, subgenotype VIIa, VIIh and VIIi. Comparing the genetic characteristics with the vaccine strains LaSota, B1 and VG/GA demonstrated that the vaccine strains had a range of 74% to 82% similarity with the partial F-gene sequences of the Philippine NDV isolates. Evolutionary divergence revealed that the Philippine NDV isolates were closely related up to 99% similarity to the ones currently circulating in Southeast Asia and the Middle East and may be a part of the emerging fifth ND panzootic.

Language

English

Location

UPLB College of Veterinary Medicine (CVM)

Call Number

LG 993.5 2016 V4 L67

Document Type

Thesis

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