Immunohistochemistry test of brain and salivary gland paraffin sections using the anti-rabies N and anti-rabies P protein polyclonal antibody for post- mortem rabies detection in dogs

Date

6-2016

Degree

Doctor of Veterinary Medicine

College

College of Veterinary Medicine (CVM)

Adviser/Committee Chair

Maria Amelita C. Estacio

Abstract

The fluorescent antibody test is the gold standard in postmortem rabies virus antigen (RVA) detection however, it has limited use in resource-limited countries. Multiple studies support immunohistochemistry (IHC) test RVA detection in formalin-fixed tissues under a light microscope. This IHC study was undertaken to compare the accuracy, sensitivity and specificity between anti- rabies N and anti-rabies Pprotein polyclonal antibody (PAb) as primary antibody in brain and salivary glands sections against Philippine RVA isolates. In 14 archived dog brain and salivary gland samples, 86%of the brain samples tested positive and 14% were negative using dFAT. IHC using anti-NPAb detected RVA in 79% of brain, 57% of mandibular and 29% of parotid samples while anti-P PAb positively detected 86% of brain, 71% of mandibular and 29% of parotid samples. Overall findings conclude that both anti-N and anti-P PAb can detect RVA in brain and salivary gland samples. Anti-P PAb was found to be more sensitive and accurate, while anti-N PAb was more specific. Brain samples were confirmed to be most ideal tissue for IHC examination compared with salivary gland tissues, with the mandibular as the preferred sample over the parotid gland.

Language

English

Location

UPLB College of Veterinary Medicine (CVM)

Call Number

LG 993.5 2016 V4 C43

Document Type

Thesis

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