Research internship on the application of microorganisms as biotechnological tool in food analysis
Date
6-2016
Degree
Bachelor of Science in Agricultural Biotechnology
College
College of Agriculture and Food Science (CAFS)
Adviser/Committee Chair
Lotis E. Mopera
Abstract
The research internship was divided into two parts. The first part of the internship involved handling of toxins and banana plant virus at the National Immunological Testing Laboratory (NITL)..The intern was assigned on the detection of aflatoxin and purification of Banana Bunchy Top Virus.Aflatoxin detection was done by running the samples via Thin Layer Chromatography using Silica Gel GF254. Purification of virus involved multiple centrifugation and reagents to separate virus from impurities.The second part of the internship aimed to determine the prebiotic potential of ube (DioscoreaalataL.) to a commercially available probioticBacillus clausii (commercial probiotic isolate) and Escherichia coliwere grown on M9 medium with ubeas treatment while glucose as the control.Cell densities reported as Log CFU ml-1were compared after 24 hours incubation at 37°C. AflatoxinG1 appeared green while B1 appeared blue-violet when viewed under UV at 366 nm wherein the latter travelled further in TLC.No virus was purified during the internship with possible sources of error are human and protocol errors.Bacillus clausii had 12% higher cell density in M9 ube compared to M9 glucose while Escherichia coli had a 10% decrease.At α=0.5, the data obtained are statistically different thus ubeis an effective prebiotic for commercially available probiotic.
Language
English
Location
UPLB Main Library Special Collections Section (USCS)
Call Number
LG 993 2016 A127 /P37
Recommended Citation
Park, Darwin C., "Research internship on the application of microorganisms as biotechnological tool in food analysis" (2016). Undergraduate Theses. 4884.
https://www.ukdr.uplb.edu.ph/etd-undergrad/4884
Document Type
Thesis
Notes
Major in Food Biotechnology