Research internship on the application of microorganisms as biotechnological tool in food analysis

Date

6-2016

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Lotis E. Mopera

Abstract

The research internship was divided into two parts. The first part of the internship involved handling of toxins and banana plant virus at the National Immunological Testing Laboratory (NITL)..The intern was assigned on the detection of aflatoxin and purification of Banana Bunchy Top Virus.Aflatoxin detection was done by running the samples via Thin Layer Chromatography using Silica Gel GF254. Purification of virus involved multiple centrifugation and reagents to separate virus from impurities.The second part of the internship aimed to determine the prebiotic potential of ube (DioscoreaalataL.) to a commercially available probioticBacillus clausii (commercial probiotic isolate) and Escherichia coliwere grown on M9 medium with ubeas treatment while glucose as the control.Cell densities reported as Log CFU ml-1were compared after 24 hours incubation at 37°C. AflatoxinG1 appeared green while B1 appeared blue-violet when viewed under UV at 366 nm wherein the latter travelled further in TLC.No virus was purified during the internship with possible sources of error are human and protocol errors.Bacillus clausii had 12% higher cell density in M9 ube compared to M9 glucose while Escherichia coli had a 10% decrease.At α=0.5, the data obtained are statistically different thus ubeis an effective prebiotic for commercially available probiotic.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993 2016 A127 /P37

Notes

Major in Food Biotechnology

Document Type

Thesis

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