Detection of goat DNA for species authentication of meat and meat products using goat-specific primers from mitochondrial cytochrome B gene

Date

6-2016

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Gedeun N. Yebron Jr.

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

A meat verification method was developed for raw, processed, putrefied and cooked meat samples. Raw meat sample extracts were used for the amplification of full-length mitochondrial cytochrome b gene of goat and sheep with a product size of 1,424bp. The amplicons were cloned and then sent for DNA sequencing. BLAST-N analysis of the obtained gene sequences of goat and sheep has shown 100% and 99% homology, respectively. The goat and sheep cytochrome b gene clones will also serve as positive control for the developed protocol. Matsunaga et al. (1999) primers were tested using the four meat sample types for effective amplification of goat-specific and sheep-specific DNA fragments having product sizes of 157bp and 331bp, respectively. The specificity of the primers was tested and results showed that the goat primers were considered goat-specific when tested to fourteen different species, while sheep primers were regarded to be faulty primers, cross-amplifying non-target goat DNA. Thus, new sheep-specific primers were designed on the more variable region of the gene and with shorter product size of 172bp than the amplicon size of the published sheep primers.The optimized verification method and the designed sheep-specific primers have great importance for the detection of frauds, and consequently for the authentication of meat and meat products.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2016 A127 /P38

Notes

Major in Animal Biotechnology

Document Type

Thesis

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