In Ovo supplementation of commercially available antioxidant and its effect on chorioallontoic membrane, embryonic growth and putative plasma vitellogenin of Philippine Mallard (Anas platyrynchos domesticus L.)

Date

6-2016

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Renato SA. Vega

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

The study was conducted to determine the effects of antioxidants in ovo on the embryonic growth and putative plasma vitellogenin (VTG) of Philippine mallard. Concentration of heavy metals like Pb and Cd were identified in eggs. Candidate antioxidants namely Vitamin C, Mega Antioxidant® and Proflavanol C® were chosen to be the treatments along with positive and negative controls. A total of 160 fertile eggs acquired in a commercial farm were arranged in Randomly Complete Block Design wherein initial egg weight was the blocking factor incubated at different incubator levels. Embryonic weight, and body measurements - i.e. head diameter, and body length were taken on 7, 14, 21 day incubation using electronic weighing scale and ImageJ®, respectively. Blood plasma from each treatment with four replicates was sampled for putative VTG determination using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). The eggs showed presence of heavy metal Cd (0.06 ppm) and Pb (1.64 ppm), expected to act as transition metals for oxidation. Embryonic weight, body length, and head diameter showed no significant differences among all treatment indicating that Antioxidant has no deleterious effects on embryo. No distinct macroscopic abnormalities were found in embryo as well as in liver. The 175 kDa Vitellogenin, an indicator for endocrine disruption was present in all treatments except for Mega Antioxidant® (a combination of several natural sources of antioxidants) implying that it protected the liver from the action of metal ions for VTG synthesis during embryonic growth until hatched.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2016 A127 /N37

Notes

Major in Animal Biotechnology

Document Type

Thesis

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