Analysis of morphometric traits and mitochondrial cytochrome oxidase I (CO1) DNA sequence of eggplant fruit and shoot borer, leucinodes orbonalis guenee (Lepidoptera: Crambidae) in Laguna, Philippines

Date

6-2016

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Desiree M. Hautea

Abstract

Eggplant fruit and shoot borer (EFSB), Leucinodes orbonalisGuenée, were collected from three eggplant growing sites in Laguna, namely: Los Baños (LB), San Pablo (SP), and Victoria (VIC). The EFSB were characterized and compared using morphological traits (five male genital structures) and sequence analysis of mitochondrial DNA cytochrome oxidase I (COI) gene. Both morphological and molecular analyses confirmed that all EFSB samples from Laguna belong to L. orbonalis species. Statistical analysis of five genital traits indicated no significant difference between EFSB-LB and EFSB-SP but differed from EFSB-VIC in all traits measured. However, the differences were very small to merit consideration as a different species. The mtDNA COI gene I sequence analysis revealed (1) three haplotypes for Laguna, (2) little overall mean distance among and within the Laguna population and (3) very high homology to previously reported L. orbonalis sequences from India where the insect must have originated and introduced to the Philippines by trade. The findings in this study can help in the development of the most cost-effective management method to control EFSB in the country.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2016 A127 /M68

Notes

Major in Plant Breeding

Document Type

Thesis

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