Development of gene specific markers for detection of cassava (Manihot esculenta Crantz) witches? broom disease as baseline for selection of host resistance
Date
6-2016
Degree
Bachelor of Science in Agricultural Biotechnology
College
College of Agriculture and Food Science (CAFS)
Adviser/Committee Chair
Antonio G. Lalusin
Restrictions
Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.
Abstract
Detection of Cassava Witches Broom Disease (CWBD) among 36 cassava accessions was done by developing seven (7) gene specific markers and using a universal group-specific primer. Five (5) developed gene specific marker were optimized and three (3) markers from the optimized gene specific marker ? CaWB1, CaWB4, and CaWB6, can detect CWBD. Further test for CaWB6 is needed to validate its detection for CWBD while CaWB2 and CaWB5 needs further optimization. Developed primers CaWB3 and CaWB7 showed amplifications on several accessions but no bands were detected on positive control. After screening all primers across samples, results show that Accession 124 (Acc 124) and Sultan 2 are putatitve susceptible lines while NSIC Cv-21 and Sultan 11 are putative resistant lines recommended for further cassava line development, serving as baseline for resistance to CWBD.
Language
English
Location
UPLB Main Library Special Collections Section (USCS)
Call Number
LG 993.5 2016 A127 /F67
Recommended Citation
Foronda, Johnric Paolo F., "Development of gene specific markers for detection of cassava (Manihot esculenta Crantz) witches? broom disease as baseline for selection of host resistance" (2016). Undergraduate Theses. 4895.
https://www.ukdr.uplb.edu.ph/etd-undergrad/4895
Document Type
Thesis
Notes
Major in Crop Biotechnology