Development of gene specific markers for detection of cassava (Manihot esculenta Crantz) witches? broom disease as baseline for selection of host resistance

Date

6-2016

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Antonio G. Lalusin

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

Detection of Cassava Witches Broom Disease (CWBD) among 36 cassava accessions was done by developing seven (7) gene specific markers and using a universal group-specific primer. Five (5) developed gene specific marker were optimized and three (3) markers from the optimized gene specific marker ? CaWB1, CaWB4, and CaWB6, can detect CWBD. Further test for CaWB6 is needed to validate its detection for CWBD while CaWB2 and CaWB5 needs further optimization. Developed primers CaWB3 and CaWB7 showed amplifications on several accessions but no bands were detected on positive control. After screening all primers across samples, results show that Accession 124 (Acc 124) and Sultan 2 are putatitve susceptible lines while NSIC Cv-21 and Sultan 11 are putative resistant lines recommended for further cassava line development, serving as baseline for resistance to CWBD.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2016 A127 /F67

Notes

Major in Crop Biotechnology

Document Type

Thesis

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