Microbial degradation of 2,4-dichlorophenoxyacetic acid by plant growth promoting bacteria (PGPB) and the effectivity of BioGroeᵀᴹ in crop production

Date

12-2015

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Katherine Ann C. Israel

Abstract

The research internship was undertaken for the trainee to observe, learn and employ microbial and chemical techniques in two experiments, wherein the first was to determine the effectivity of plant growth promoting bacteria (PGPB) in utilizing 2,4-dichlorophenoxy acetic acid as its sole carbon source, while the second experiment was to determine BioGroeᵀᴹ's effectivity on crop production. The internship was conducted at the National Institute of Molecular Biology and Biotechnology (BIOTECH), University of the Philippines Los Baños (UPLB) and at Costales Nature Farms, Majayjay, Laguna, respectively, from January to July 2015. The commodities used as experiment units in the first experiment was PGPB 1, PGPB 2, and PGPB 4. Meanwhile for the second experiment, five experimental units were planted as commodities for the BioGroeᵀᴹ effectivity namely Romaine lettuce (Lactuca sativa), kale (Brassica oleracea), Japanese cucumber (Cucumis sativus), French beans (Phaseolus vulgaris), and D-MAX tomato (Solanum lycopersicum). PGPB 1, 2 and 4 were grown in synthetic minimal medium containing 2,4-D. Afterwards, the enrichment was autoclaved at 121°C. A loopful of the enrichment was then streaked in tryptone soy agar (TSA) medium containing 2,4-D as well as the pure cultures of the three plant growth promoting bacteria followed by storage at 37°C. Bacterial growth was observed after 24 hours and gram stained to determine gram positivity or negativity of the bacteria. While the commodities in the BioGroeᵀᴹ experiment was prepared by sowing the seeds in seedling trays under different treatments namely control (H₂O), Effective Microorganisms Activated Solution (EMAS), BioGroeᵀᴹ and EMAS with BioGroeᵀᴹ. The crops' germination rate, height and weight were obtained. The trainee was able to learn and employ various laboratory, planting and statistical techniques which are vital in her future career as researcher.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993 2015 A127 /C68

Notes

Major in Food Biotechnology

Document Type

Thesis

This document is currently not available here.

Share

COinS