Optimization of tissue culture requirements for in vitro shoot multiplication of garlic (Allium sativum L.)

Date

6-2016

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Lilian F. Pateña

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

A study was conducted in order to optimiza the garlic shoot multiplication protocol currently used by the Plant Cell and Tissue Culture Laboratory (PCTCL),Institute of Plant Breeding (IPB,) to be able to reduce total production cost.Shoot tip explants of three accessions (Ilocos White, Iloilo, Batanes) were cultured using the established garlic induction medium (GIM)and four test media The latter media contain either Schenk and Hildebrandt (SH) or Murashige and Skoog (MS) basal salts and additives and 2-isopentenyladenine (2-ip) and 6-benzlaminopurine (BAP) as growth regulators.A significant statistical difference among the test media was observed. Using the mean number of shoots, SH2 (control, SH with 2-ip, μ=2.71) was significantly different from SHB (SH with BAP, μ=1.29), MS with 2-ip (μ=1.35) and MS with BAP (μ=0.04).The highest number of shoots observed among treatments was 16 and 1 the lowest. Due to hyperhydricity, the cultures on MS with BAP had the highest mortality rate (95%) across all accessions. Based on the number of shoots formed, there was no significant difference among accessions The PCTCL protocol used GIM is routinely used for shoot induction of explants prior to shoot multiplication. However, results showed that the cultures formed multiple shoots even without using GIM. This study confirmed that SH medium with 2-ip is the best for shoot multiplication and that the cost of production can be reduced with the deletion of the GIM step.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2016 A127 /B36

Notes

major in Crop Biootechnology

Document Type

Thesis

This document is currently not available here.

Share

COinS