Serological and molecular detection, and transmission of the virus causing leaf curl disease in tobacco (Nicotiana tabacum L.)

Date

12-2015

Degree

Bachelor of Science in Agriculture

Major Course

Major in Plant Pathology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Filomena C. Sta. Cruz

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

In the Philippines, the leaf curl disease of tobacco has been identified based on the similarity of the symptoms with tobacco leaf curl and on the transmission of the virus by the whitefly (Bemisi tabaci) vector. This study aimed to identify the virus causing tobacco leaf curl observed in Claveria, Misamis Oriental and Los Baños, Laguna through serological and molecular detection, bioassay and virus transmission test. Leaf samples collected in tobacco plants from three locations in Claveria, Misamis Oriental were positive in PCR assay for begomovirus infection. The virus was detected by polymerase chain reaction (PCR) using three sets of begomovirus degenerate primers designed for the DNA-A component of the viral genome, namely TY1F/TY1R, PALv1978/PAR1c715 and PALv1978/PAR1c496 with amplicons of about 585 bp, 1500 bp and 400 bp in size, respectively. Two of 29 samples from Claveria reacted positively to Tobacco mosaic virus (TMV) infection in enzyme-linked immunosorbent assay. However, these samples have negative reaction in bioassay for TMV and Cucumber mosaic virus (CMV) infection using the indicator plants, Nicotiana tabacum and N. glutinosa. The virus was successfully transmitted by the whitefly vector using the isolates from Los Baños, Laguna. Thus, the virus causing tobacco leaf curl disease in Claveria, Misamis Oriental and Los Baños, Laguna is a Begomovirus.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2015 A3 /B46

Document Type

Thesis

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