Efficacy of ascorbic acid (vitamin C) against Escherichia coli in mangoes (Mangifera indica) and Escherichia coli monitoring in ready-to-eat mangoes ( Mangifera indica) sold at salad bars inside malls in Laguna

Date

12-2015

Degree

Bachelor of Science in Food Technology

Major Course

Major in Food Science

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Bryan Rey R. Oliveros

Abstract

Fresh-cut fruit production is becoming more common and important because consumers are becoming more health-conscious and have lesser time for food preparation. Ready-to-eat fruits especially mangoes are popular in supermarket salad bars inside malls. Food safety awareness in the Philippines is one of the primary concerns of the Department of Health and safety of the supermarket salad bars is important since it is regularly consumed by the public. Because of this, detection of E. coli in salad bars was done in seven malls in Laguna for five months (January-May, 2015). Results showed that the fresh-cut mangoes are unsatisfactory. In need of an antimicrobial for the fresh-cut mangoes, the antimicrobial activities of ascorbic acid, vanilla, cinnamon, clove, and allspice against E. coli were screened. Five percent ascorbic acid showed a significant zone of inhibition. Determination of ascorbic acid's MIC through tube dilution and MBC by streak-plating was done. MIC was found to be 0.3% and MBC was found to be 0.6%. Three concentrations (2.5%, 1.25%, 0.6%) of ascorbic acid were used as dips for artificially inoculated fresh-cut mangoes to determine the kill rate and log reduction of ascorbic acid against E. coli. Results show that 2.5% ascorbic acid had the highest kill rate (71.81%) and log reduction (5.7447). It can be deduced that the concentration of ascorbic acid, and kill rate and log reduction are directly proportional. The study showed that ascorbic acid is effective in inhibiting E. coli on fresh-cut mangoes.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2015 F61 /T54

Document Type

Thesis

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