Comparative performance of flocculating and non-flocculating strains of Saccharomyces cerevisiae in chemostat culture

Date

6-2016

Degree

Bachelor of Science in Chemical Engineering

College

College of Engineering and Agro-Industrial Technology (CEAT)

Adviser/Committee Chair

Jovita L. Movillon

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

A continuous flow process of fermentation was studied using a flocculating (TB3) and a non-flocculating (2030) strains of Saccharomyces cerevisiae in an improvised bioreactor at a working volume of 175 ml anaerobic conditions for 153 hours. Molasses medium was used for inoculum build-up while sugarcane juice was used as the fermentation medium. It was found out that for TB3 and 2030, the residual sugar consumption, ethanol, and total cell concentration exhibited similar trend. Starting at low concentrations, they continuously increased until a peak is reached, and then gradually decreased and stabilized at a nearly fixed value. However, fluctuations in the residual sugar of 2030 at the early phase of fermentation was observed due to pump malfunctions. The maximum volumetric ethanol productivity was 1.89 g/L h for TB3 and 4.07 g/L h for 2030. The considerable low productivity values obtained from the beer samples of TB3 could be due to clumping or aggregation at the base of the fermenter. Statistical analyses via two-tailed T-test showed that at 95% confidence interval, there is no significant difference on the residual sugat, ethanol, and productivity between the two strains, however, the total cell concentration showed otherwise. Based on the fermentation parameters obtained, it can be said that a higher ethanol productivity was achieved in a continuous flow fermentation using free cells. As calculated in material balance and preliminary costing, the scale-up of the process is not yet feasible considering only the ethanol selling price as basis.

Language

English

Location

UPLB College of Engineering and Agro-Industrial Technology (CEAT)

Call Number

LG 993.5 2016 E62 /P38

Document Type

Thesis

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