Parametric and optimization study on the enzymatic extraction of pectin from mango peels
Date
6-2016
Degree
Bachelor of Science in Chemical Engineering
College
College of Engineering and Agro-Industrial Technology (CEAT)
Adviser/Committee Chair
Jovita L. Movillon
Restrictions
Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.
Abstract
Utilization of by-product such as mango peels in the production of valuable product like pectin is one of the emerging concern nowadays. The enzymatic way of producing pectin is a promising step in lessening the environmental impact such as pollution and toxicity due to chemicals. For this study, the enzyme used to liberate the pectin was Cellulase (Sigma C-9748). The effects of the three factors (solids loading, enzyme loading and reaction time) on the responses (pectin yield and galacturonic acid yield) were determined significant through the two-level factorial design. Response- surface methodology (Box-Behnken) showed statistically significant quadratic model. Based on the numerical optimization, the predicted pectin yield (20.01 % w/w) and galacturonic acid yield (147.711 mg/g) shall be obtained at 2 % (w/v) solids loading, 3.598 % (w/w) and 11.562 (hour) reaction time. The experimental verification of optimum conditions 2 % (w/v solids loading, 3.599 % w/w enzyme loading and 11.561-hour reaction time) showed a pectin yield of 19.59 % (w/v) and a galacturonic acid yield of 146.307 mg/g. The predicted and actual values were compared and the perfect difference for pectin yield was 2.10 % and 0.95 ^ for galacturonic acid.
Language
English
Location
UPLB College of Engineering and Agro-Industrial Technology (CEAT)
Call Number
LG 993.5 2016 E62 /B66
Recommended Citation
Bondoc, Yves D., "Parametric and optimization study on the enzymatic extraction of pectin from mango peels" (2016). Undergraduate Theses. 5430.
https://www.ukdr.uplb.edu.ph/etd-undergrad/5430
Document Type
Thesis