Parametric and optimization study on the enzymatic extraction of pectin from mango peels

Date

6-2016

Degree

Bachelor of Science in Chemical Engineering

College

College of Engineering and Agro-Industrial Technology (CEAT)

Adviser/Committee Chair

Jovita L. Movillon

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

Utilization of by-product such as mango peels in the production of valuable product like pectin is one of the emerging concern nowadays. The enzymatic way of producing pectin is a promising step in lessening the environmental impact such as pollution and toxicity due to chemicals. For this study, the enzyme used to liberate the pectin was Cellulase (Sigma C-9748). The effects of the three factors (solids loading, enzyme loading and reaction time) on the responses (pectin yield and galacturonic acid yield) were determined significant through the two-level factorial design. Response- surface methodology (Box-Behnken) showed statistically significant quadratic model. Based on the numerical optimization, the predicted pectin yield (20.01 % w/w) and galacturonic acid yield (147.711 mg/g) shall be obtained at 2 % (w/v) solids loading, 3.598 % (w/w) and 11.562 (hour) reaction time. The experimental verification of optimum conditions 2 % (w/v solids loading, 3.599 % w/w enzyme loading and 11.561-hour reaction time) showed a pectin yield of 19.59 % (w/v) and a galacturonic acid yield of 146.307 mg/g. The predicted and actual values were compared and the perfect difference for pectin yield was 2.10 % and 0.95 ^ for galacturonic acid.

Language

English

Location

UPLB College of Engineering and Agro-Industrial Technology (CEAT)

Call Number

LG 993.5 2016 E62 /B66

Document Type

Thesis

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