Detection of Salmonella spp. from cloacal swabs, ileal and cecal contents in native chickens (Paraoakan x Banaba Crossbreed) grown under backyard production system in Laguna, Philippines

Date

2022

Degree

Doctor of Veterinary Medicine

Abstract

Salmonella has been one of the most recognized enteric pathogens globally. It has a public health significance considering the resistance of many Salmonella serovars to antibiotics and the prevalence of Salmonella infections, which may vary among humans and animals. The said infection can be acquired through handling of raw contaminated meats, exposure to infected animals, live vectors and contaminated farm. In this study, Philippine native chickens were obtained from five selected backyard production systems in Los Baños, Laguna. The isolation of Salmonella spp. was achieved by conventional culture methods and PCR through collecting samples from the ileum, cecum and cloaca of Philippine native chickens, which were acquired for this significant study. The overall detection rate of Salmonella by conventional culture method was 64.67% (193/300), of which 25% (25/100) was isolated from cloacal swab samples; ileal contents with 71% (71/100) and cecal content samples with 98% (98/100). The overall detection rate of Salmonella by PCR was 15.57% (7/45) from days 30, 90, and 150. Only Typhimurium was specifically identified by gel electrophoresis at day 30. The present study will inform the backyard sectors and raisers to monitor the management of backyard poultry production systems in the Philippines.

Language

English

Location

UPLB College of Veterinary Medicine (CVM)

Notes

Award: Teodulo C. Topacio, Sr. Award for Best Undergraduate Thesis for Microbiology

Document Type

Thesis

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