Hepatic Vitellogenin mRNA Expression in Male Silver Perch (Leiopotherapon Plumbeus) from Calibato, Yambo, Bunot, and Mohicap Lakes of San Pablo, Laguna

Date

2022

Degree

Doctor of Veterinary Medicine

College

College of Veterinary Medicine (CVM)

Abstract

Calibato, Yambo, Bunot, and Mohicap Lakes of San Pablo were evaluated to detect the occurrence of endocrine disruptors (EDs) in water samples and their effects in fish. Adult male ayungin were caged in each lake and liver samples were analyzed to detect the expression of three vitellogenin (VTG) mRNA (VTG A, B, and C) using real-time polymerase chain reaction (RT-qPCR) and spectrophotometry. Results showed induction of all VTG gene types in most fish samples. Liver samples from Bunot Lake had significantly higher percentage of fish that expressed VTG A in comparison to the reference group. On the other hand, fish from Mohicap, Calibato, and Yambo Lakes did not express VTG A at all. Conversely, samples from each lake expressed VTG B and VTG C. Enzyme linked immunosorbent assay of water samples revealed detectable levels of 17beta-estradiol (E2) and bisphenol A (BPA) in all sites, which suggests the role of these chemicals in inducing VTG mRNA expression in fish. Due to the potential of EDs to cause aberrations in the development, growth, and reproduction of vulnerable organisms such as fish, the formulation of measures to prevent or reduce ED contamination of the lakes is recommended.

Language

English

Notes

Award: Dr. Elito F. Landicho Memorial Award for Best Undergraduate Thesis in Veterinary Physiology

Document Type

Thesis

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