Alkali-mediated delignification and simultaneous saccharification and fermentation of itch grass (Rottboellia conchinchinensis) for cellulose ethanol production

Date

4-2010

Degree

Bachelor of Science in Chemical Engineering

College

College of Engineering and Agro-Industrial Technology (CEAT)

Adviser/Committee Chair

Marilyn C. Del Barrio

Co-adviser

Veronica P. Migo

Committee Member

John Meynard M. Tengco, Monet Concepcion C. Maguyon

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Abstract

This study explored the possibility of itch grass, Rottboellia conchinchinesis, as a candidate feedstock for ethanol production. Alkali pretreatment was optimized to account for itch grass 15.38% lignin content using different NaOH concentrations (0.5%, 1.5%, 2.5% w/v), reaction time (20,40, 60 minutes) and temperatures (60°C, 80°C, 100°C) as treatment factors. A Box-Benhken design was applied to generate experimental set-ups using these treatment factors, with total sugar concentration (%) as the response variable for the indirect index for delignification. After pretreatment, numerical optimization obtained the optimum sugar yield in the liquid phase of 27.31% using 2.50% w/v NaOH concentration, 60 minutes and 100°C. Ethanol production was assessed via simultaneous saccharification and fermentation (SSF). Liquozyme SC DS with 113 FPU/ml cellulose activity was used for enzymatic hydrolysis, while the industrial yeast strains Saccharomyces cerevisiae HBY3 and Scheffersomyces stipilis Y-7124 were used for the fermentation of glucose and xylose, respectively. The maximum ethanol concentration of 0.43% v/v was obtained, but can still be enhanced if further optimization in the fermentation process is conducted.

Language

English

Location

UPLB College of Engineering and Agro-Industrial Technology

Document Type

Thesis

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