Butanol production via glycerol fermentation by Clostridium pasteurianum

Marco R. Lao

Date

3-2010

Degree

Bachelor of Science in Chemical Engineering

College

College of Engineering and Agro-Industrial Technology (CEAT)

Adviser/Committee Chair

Jovita L. Movillon

Co-adviser

Veronica P. Migo

Committee Member

Lorele C. Trinidad, Manolito E. Bombase, John Meynard M. Tengco

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Abstract

The increasing production of biodiesel resulted in an increase in the supply of glycerol in the world market. The potential of glycerol as a substrate for the production of butanol via fermentation by Clostridium pasteurianum was studied. Glycerol fermentation resulted in the production of butanol as the major product with the co-production of ethanol and trace amounts of acetic and butyric acid. 1,3-propanediol was not detected in any of the culture conditions tested. A slightly acidic pH of 5 - 6 gave the highest butanol concentrations of up to 7.07 g/L. Ethanol production was higher at higher pHs. Supplementation of the medium with phosphate buffer at pH 6.0 showed improved glycerol consumption and butanol production. Cultures supplemented with 65mM at pH 6.0 gave a yield of 0.4078 g butanol/g glycerol consumed. A strong positive correlation existed between biomass formation, butanol production and gas evolution. C. paseturianum was able to ferment biodiesel-derived glycerol when treated with 85% (w/w) phosphoric acid till pH 4 producing up to 2.21 g/L butanol.

Language

English

Location

UPLB College of Engineering and Agro-Industrial Technology

Call Number

LG 993 2010 E62 L36

Document Type

Thesis

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