Effect of culture conditions on pigment production of aspergillus niger in liquid medium

Abstract

© 2018, College of Agriculture and Food Science, University of the Philippines Los Banos. All rights reserved. Colorants may be synthetic or natural. Approved natural colorants for human consumption are mostly plant in origin but production is highly dependent on external supply of raw materials and requires vast areas of land for cultivation. An alternative is to produce natural colorants from microorganisms such as Aspergillus niger. Pigment production of Aspergillus niger was evaluated using five different parameters: inoculum concentration, fermentation medium, initial pH of medium, incubation temperature, and incubation period. Corresponding amount of pigment produced in the fermentation broth (crude pigment) at wavelengths of 396, 407, and 485 nm were measured through absorbance using a spectrophotometer. Under the best culture conditions, these pigments were extracted and dried, then the amount of concentrated pigment was computed by dividing the weight of dried concentrated pigment by the volume of fermentation broth containing the extracellular pigment. Culture conditions, described as follows, resulted in the highest amount of crude pigment in Aspergillus niger: potato dextrose broth (9.2195–11.9110 mg/mL) as fermentation medium with an initial pH of 6.0 (8.4440–12.1700 mg/mL) using 1% inoculum (9.442–13.0410 mg/mL) and incubated at 27 °C (9.4425–13.0410 mg/mL) for 7 d (9.4425–13.0410 mg/mL). The amount of concentrated pigment obtained under these conditions was 1.21 mg per mL of the fermentation broth.

Source or Periodical Title

Philippine Agricultural Scientist

ISSN

317454

Page

176-184

Document Type

Article

Subject

Aspergillus niger, Concentrated pigment, Crude pigment, Culture conditions, Pigment production

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